Vancomycin-A resistant Enterococcus (VRE) is the second-leading cause of drug-resistant nosocomial infections. This assay enables detection of the vanA gene at 2 different sites in the E. faecium vanA operon. This assay contains flanking primers and 2 iDDS probe/antiprobe pairs labeled for detection in the HEX and ROX channels.
The iDDS probes can detect the vanA gene in human DNA samples, while the excess matching quencher-labeled antiprobes prevent false-positive detection of the related vanB gene or vancomycin-sensitive Enterococcus or strains.
Kodiak Taq HotStart Master Mix:
GeneTAG is offering a preferred enzyme master mix that is sold our probe kit customers. However, other qPCR Taq enzymes are suitable for our probes.