Advanced qPCR probes

GeneTAG Technology, Inc.


Channel

FAM


ROX


FAM


FAM


ROX


ROX


​FAM, HEX,

and ROX


FAM, HEX, ROX, Cy5


​Product information for MTB assays:


MTB iDDS probe target

16S rRNA


WT inhA (-15C, -8T)


​inhA -15C>T mutant


inhA, -8T>A mutant


WT katG (944G)


katG, 944G>C mutant


​​WT rpoB codons 516, 526, and             531​


WT rpoB codons 511–513, 516,           526, and 531



Companion enzyme master mix:


Description

Kodiak Taq HotStart Master Mix, including dNTPs, MgCl2, and buffer



Tiered pricing available


Limitations of use: Research use only



Catalog #

G1200

​​

G1250


G1251


G1254


​G1252


G1253


​G1300



G1301






Catalog #

​G4000

Product descriptions:

MTB 16S rRNA probe

Specific detection of 16S rRNA transcripts enables discernment between active and latent Mycobacterium tuberculosis ​ (MTB) infections  and provides a basis for MTB drug-resistance profiling. Our MTB 16S rRNA iDDS probe assay  (G1200) discriminates against 16S rRNA orthologs from other species (right panel). As shown, the iDDS probe provides single-base discrimination over a wide annealing temperature range.

iDDS probe assays for detecting MTB 16S rRNA and drug-resistant MTB mutants

Rifampicin-resistance MTB probes

We offer a multiplex iDDS probe assay (G1300) containing 3 iDDS probes specific for WT sequences at rpoB codons 516, 526, and 531. The probes do not detect the most commonly occurring rifampicin (RIF)-related mutations occurring at these sites, thus enabling negative detection of RIF-resistant MTB strains, as shown below. We also offer a 4-color multiplex assay (G1301) for negative detection of RIF-resistance mutations at rpoB codons 511–513, 516, 526, and 531.       

​​​​Isoniazid-resistance MTB probes

Approximately 90% of isoniazid (INH)-resistant MTB strains harbor mutations in the inhA and katG genes. We have developed iDDS probes to detect inhA variants governing INH resistance. The WT inhA assay (G1250) discriminates against the 2 most common INH-resistance mutations in inhA (-15C>T and -8T>A). Similarly, the -15C>T assay (G1251) and -8T>A assay (G1254) discriminates against WT and off-target mutant sequences. We also offer iDDS probes for detecting the katG 944G>C mutant conferring INH resistance and WT 944G katG.


Price

On request





















Price

On request


# of 20-uL

reactions

50–200


50–200


50–200


50–200


50–200


50–200


50–200



50–200





# of 20-uL

reactions

50–200