Kodiak Taq HotStart Master Mix, including dNTPs, MgCl2, and buffer
Tiered pricing available
Limitations of use: Research use only
MTB 16S rRNA probe
Specific detection of 16S rRNA transcripts enables discernment between active and latent Mycobacterium tuberculosis (MTB) infections and provides a basis for MTB drug-resistance profiling. Our MTB 16S rRNA iDDS probe assay (G1200) discriminates against 16S rRNA orthologs from other species (right panel). As shown, the iDDS probe provides single-base discrimination over a wide annealing temperature range.
iDDS probe assays for detecting MTB 16S rRNA and drug-resistant MTB mutants
We offer a multiplex iDDS probe assay (G1300) containing 3 iDDS probes specific for WT sequences at rpoB codons 516, 526, and 531. The probes do not detect the most commonly occurring rifampicin (RIF)-related mutations occurring at these sites, thus enabling negative detection of RIF-resistant MTB strains, as shown below. We also offer a 4-color multiplex assay (G1301) for negative detection of RIF-resistance mutations at rpoB codons 511–513, 516, 526, and 531.
Isoniazid-resistance MTB probes
Approximately 90% of isoniazid (INH)-resistant MTB strains harbor mutations in the inhA and katG genes. We have developed iDDS probes to detect inhA variants governing INH resistance. The WT inhA assay (G1250) discriminates against the 2 most common INH-resistance mutations in inhA (-15C>T and -8T>A). Similarly, the -15C>T assay (G1251) and -8T>A assay (G1254) discriminates against WT and off-target mutant sequences. We also offer iDDS probes for detecting the katG 944G>C mutant conferring INH resistance and WT 944G katG.