GeneTAG Technology, Inc.

Kodiak Taq HotStart Master Mix:

GeneTAG is offering a preferred master mix that is sold to our probe kit customers. However, other qPCR Taq enzymes are suitable for our probes.



Price

On request





Price

​On request



Channel

FAM


ROX



Description

influenza A ZIPR probe assay


influenza B ZIPR probe assay




Description

Kodiak Taq HotStart Master Mix, including dNTPs, MgCl2, and buffer


# of 20-uL

reactions

50–200


50–200



​# of 20-uL

reactions

50–200

Product information:


Catalog #

G1150


G1151


Companion enzyme master mix:


Catalog #

G4000



Tiered pricing available


Limitations of use: Research use only

Product description:

The accurate detection and discrimination of influenza A and influenza B viruses is important for primary research and clinical treatment. We have developed ​a ZIPR probe assay for influenza A that detects the viral M1 gene of various strains (pandemic 2009 H1N1, old H1N1, H3N2, H5N1) at a highly conserved site, while discriminating against influenza B targets. Our influenza B ZIPR probe detects a conserved site in the viral NS1 gene, without detecting similar sequences from influenza A strains. Both assays contain a probe/antiprobe pair and a second primer. 


The fluor-labeled ZIPR probes drive amplification of the respective viral targets, while the excess quencher-labeled antiprobes prevent false-positive amplification of related off-targets and block signaling in the absence of the viral target. Examples of the performance of these probes is shown below.P probe assay

Influenza A and B ZIPR probe assays

Advanced qPCR probes