Non-polio enteroviruses cause a diverse spectrum of human diseases. Approximately 100 non-polio enterovirus (EV) serotypes are known, encompassing all Coxsackievirus A/B, echovirus, enterovirus, and rhinovirus strains. This assay enables robust detection at a site conserved across all EV genomes. The assay contains an iDDS probe/antiprobe pair and flanking primers.
The FAM-labeled iDDS probe detects EV cDNA, while the excess quencher-labeled antiprobe prevents false-positive detection of parechoviruses (PV) or other EV-negative DNA samples.
Kodiak Taq HotStart Master Mix:
GeneTAG is offering a preferred enzyme master mix that is sold to our probe kit customers. However, other qPCR Taq enzymes are suitable with our probes.
Non-polio enteroviruses, internal DNA Detection Switch (iDDS) probe assay