Advanced qPCR probes

GeneTAG Technology, Inc.



Price

On request












Price

On request



Channel

FAM


FAM, HEX


FAM


​FAM, HEX


FAM

Product information:


Catalog #

G3301


G3302


G3351


G3352


G3400


Companion enzyme master mix:

​​

Catalog #

G4000



Tiered pricing available


Limitations of use: Research use only

Product descriptions:

GeneTAG Technology offers custom-designed assays for detecting essentially any target of interest. Internal DNA Detection Switch (iDDS) probes deliver error-checking single-base discrimination over 20–30C annealing temperature ranges, making them an excellent choice for validating Next Generation Sequencing results, studying single-nucleotide polymorphisms, or discriminating against similar targets. iDDS probes are paired with nearly complimentary quencher-labeled antiprobes that prevent binding to off-targets. Custom dual iDDS probe assays can be used for detecting alternate SNP alleles or for simultaneous 2-color detection of two separate targets of interest.


ZIPR primer-probes have a probe: antiprobe design that is similar to the iDDS probe system, although the 3' ends of ZIPR probes are not blocked and they serve as a primer-probe. This design provides a measure of error-checking when detecting gene fusions or other targets. Dual ZIPR probe assays label both ends of PCR amplicons to confirm true detection of gene fusions or other targets.


​Our custom Universal probe assays use generic components that can be provided at lower cost. Universal probes involve a 2-step amplification process. The first step extends the amplicon with the universal sequence and then all amplicons are labeled in the second step with the Universal probe.


Kodiak Taq HotStart Master Mix:

GeneTAG is offering a preferred enzyme master mix that is sold to our probe kits customers. However, other qPCR Taq enzymes are suitable for our probes.

Custom-designed assays for studying genes of interest or for validating Next Generation Sequencing results



Description

Custom single iDDS probe assay


Custom dual iDDS probe assay


Custom single ZIPR probe assay


Custom dual ZIPR probe assay


Single Universal probe assay




Description

Kodiak Taq HotStart Master Mix including enzyme, dNTPs, MgCl2, and buffer

# of 20-uL 

reactions

400


400


400


400


400



# of 20-uL

reactions

​200