This single-iDDS probe assay enables CRHR1 mRNA detection. The assay contains a reverse primer matching the CRHR1 mRNA transcript for use in reverse transcriptase reactions. For qPCR, the assay also includes a single iDDS probe/antiprobe pair and flanking primers. Importantly, the reverse primer and iDDS probe assay target conserved exons common to most CRHR1 splice variants.
The iDDS probe/antiprobe pair is labeled for detection in the ROX channel. The excess quencher-labeled antiprobe blocks false-positive detection in the absence of the intended target.
Kodiak Taq HotStart Master Mix:
GeneTAG is offering a preferred enzyme master mix that is sold to our probe kit customers. However, other qPCR Taq enzymes are suitable for our probes.