ADCYAP1R1 mRNA internal DNA Detection Switch probe assay
This single-iDDS probe assay enables ADCYAP1R1 mRNA detection. The assay contains a reverse primer matching the ADCYAP1R1 mRNA transcript for use in reverse transcriptase reactions. For qPCR, the assay also includes a single iDDS probe/antiprobe pair and flanking primers. Importantly, the reverse primer and iDDS probe assay target conserved exons common to most ADCYAP1R1 splice variants.
The iDDS probe/antiprobe pair is labeled for detection in the HEX channel. The excess quencher-labeled antiprobe blocks false-positive detection in the absence of the intended target.
Kodiak Taq HotStart Master Mix:
GeneTAG is offering a preferred enzyme master mix that is sold to our probe kit customers. However, other qPCR Taq enzymes are suitable for our probes.
Kodiak Taq HotStart Master Mix, including dNTPs, MgCl2, and buffer